cd16 apc vio770  (Miltenyi Biotec)

Journal: NPJ Precision Oncology

Article Title: Multi-cancer detection of circulating tumor cells by targeting oncofetal chondroitin sulfate

doi: 10.1038/s41698-025-00936-3

Figure Lengend Snippet: a Representative images of cancer cells (A549, ES-2 or COLO205) spiked into 1 mL of healthy donor blood followed by staining with rVAR2:dextran conjugated to PE (yellow), APC-conjugated antibodies targeting CD45/CD66b/CD16 (magenta) and DAPI (blue). Scale bar is 30 μm. b Recovery (%) of 10, 50 or 100 cancer cells spiked into 1 mL of healthy donor blood. The samples were stained and identified as described in (a).

Article Snippet: Subsequently, the samples were stained with a cocktail of PE-conjugated dextran complexed with rVAR2 (4 nM) in combination with APC-labelled antibodies targeting CD45 (1:20, Thermo Fischer, 17-0459-42, clone: HI30), CD16 (1:40, Thermo Fischer, MHCD1605, clone: 3G8), and CD66b (1:20, Miltenyi Biotec, 130-117-692, clone: REA306) in DPBS containing 2% FBS with an adjusted NaCl concentration of 300 mM (final concentration).

Techniques: Staining

Journal: NPJ Precision Oncology

Article Title: Multi-cancer detection of circulating tumor cells by targeting oncofetal chondroitin sulfate

doi: 10.1038/s41698-025-00936-3

Figure Lengend Snippet: a The number of ofCS+ CTCs detected with rVAR2:dextran in 4 mL of blood in patients with epithelial cancer (n = 25) or mesenchymal cancer (n = 3) and in healthy controls (n = 13). The samples were stained with PE-labelled rVAR2:dextran and APC-conjugated antibodies targeting CD45, CD66b and CD16. Cell nuclei were stained with DAPI. b Representative immunofluorescence images of ofCS+ CTCs from six different cancer patients. The CTCs were stained as described in ( a ). A CTC was defined as ofCS(+), CD45(−), CD66b (−), CD16(−) and DAPI(+).

Article Snippet: Subsequently, the samples were stained with a cocktail of PE-conjugated dextran complexed with rVAR2 (4 nM) in combination with APC-labelled antibodies targeting CD45 (1:20, Thermo Fischer, 17-0459-42, clone: HI30), CD16 (1:40, Thermo Fischer, MHCD1605, clone: 3G8), and CD66b (1:20, Miltenyi Biotec, 130-117-692, clone: REA306) in DPBS containing 2% FBS with an adjusted NaCl concentration of 300 mM (final concentration).

Techniques: Staining, Immunofluorescence

Journal: NPJ Precision Oncology

Article Title: Multi-cancer detection of circulating tumor cells by targeting oncofetal chondroitin sulfate

doi: 10.1038/s41698-025-00936-3

Figure Lengend Snippet: The figure shows four different morphological categories used to describe the ofCS+ CTCs detected in a blood sample from a patient with high-grade serous ovarian cancer. The sample was stained with rVAR2:dextran (yellow), CD45/CD16/CD66b (magenta) and DAPI (blue).

Article Snippet: Subsequently, the samples were stained with a cocktail of PE-conjugated dextran complexed with rVAR2 (4 nM) in combination with APC-labelled antibodies targeting CD45 (1:20, Thermo Fischer, 17-0459-42, clone: HI30), CD16 (1:40, Thermo Fischer, MHCD1605, clone: 3G8), and CD66b (1:20, Miltenyi Biotec, 130-117-692, clone: REA306) in DPBS containing 2% FBS with an adjusted NaCl concentration of 300 mM (final concentration).

Techniques: Staining